Subject:MSc Biochemistry
BCH101 - Cell biology, Microbiology and Virology.
Semester-I
CELL BIOLOGY:
Cell as a basic unit of life. Cell organization of prokaryotic and eukaryotic cells. Structural and functional compartalization of cell –mitochondria, chloroplast, lysosomes, golgi bodies, plasma membrane and cytoskeleton, cell wall, nucleus.
Cell cycle, cell division - mitosis and meiosis. Chromosome structure, gene, gene number, gene clusters and Pseudogene. Polytene and lampbrush chromosomes. Packing of DNA, supercoiled DNA, nucleosome, Inverted repeats, repetitive DNA sequence, satellite DNA. Cell trafficing.
MICROBIOLOGY:
Bacterial nomenclature and classification: Principles of bacterial nomenclature, classification of prokaryotic organisms, numerical taxonomy, chemotaxonomy, genetic and serological methods, surface properties of bacteria.
Methods in microbiology: Pure culture techniques, principles of microbial nutrition, construction of culture media, enrichment culture techniques for isolation of chemoautotrophs, chemoheterotrophs and photosynthetic microorganisms.
Sterilization-Application of sterilization methods in biotechnology, Various sterilization methods, Microbial contamination control and Sterility testing.
Microbial growth: The definition of growth, mathematical expression of growth, growth curve, measurement of growth and growth yield, synchronous growth, continuous culture.
VIROLOGY:
Classification and General properties and structure of plant, animal and bacterial viruses.
Bacteriophages – one step growth experiment, single burst and premature lysis experiments, productive cycles of ? and Fx-174 viruses, lysogeny – P1, P2, P22 and Mu1 phages, RNA phages, isolation and cultivation of bacterial viruses. Animal viruses- productive cycle of DNA viruses- parvo, adeno and SV40.
RNA viruses- reo, rhado, picorna, polio, Influenza Retrovirus (RSV and HIV)
Cultivation and enumeration of viruses- cultivation in cell culture, chick embryo and animal inoculation. Persistent chronic and acute viral infections. Plant viruses- transmission, effect on plants, common diseases, TMV.
Slow viruses and DI viruses- discovery and importance. Inhibition and inactvation of viruses by physical and chemical agents.
Interferon- types, nomenclature and classification, induction, antiviral effect, antiviral proteins- ds RNA dependent and independent pathways.
|
No responses found. Be the first to respond and make money from revenue sharing program.
|