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Posted Date: 06 Aug 2009 Posted By: Tushar kanta Rath Member Level: Bronze
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2009 Berhampur University M.Sc BioTechnology ENZYMOLOGY Question paper
1.Describe the steps for the purification of enzyme
2.Describe the mechanism of reaction catalysed by chymotrypsin.
3.Write notes on
a.Alosteric control of enzymes
b.General acid-base catalysis.
4.Derive Michaelis-Menten equation. Add a note on the significance of Km value.
5.Write notes on
a.Competitive inhibition of enzyme
b.Factors influencing enzyme kinetics
6.Describe the determination of 3-D structure of enzyme active site.
7.Write notes on the following:
a.Use of substrates analogues for the determination of active site of enzymes.
b.Enzyme catalysis in solution.
8.describe the physical methods of immobilization of enzymes and add a note on the industrial application of immoblized enzymes.
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